NormaladultkeratinocytesinKGM-Gold™BulletKit™seenunderlightmicroscopy

ProductOverview:

MostofLonza"sprimarycellsarealsoavailableas proliferatingcells ineitherflasksorplates,oraspelletsinRNALater^®.

DiseasedHumanEpidermalKeratinocytes(D-HEK) areisolatedfromhumandonorsthathavebeendiagnosedwithdiabetestypeIIdisease.Furtherdonorinformation,includingmedications,BMI,anddiseasedurationmaybeobtainedbycontactingLonzaScientificSupport.  DiseasedcellsaregrowninthesameoptimizedmediasystemasNormalHumanKeratinocytes.

SelectReferenceswithLonza’sKeratinocytes:

1)Protectivebarrier:Keratinocytes werestimulatedwithhistamineorhistaminereceptorligandstoshowtheformationofadefectiveskinbarrier. GschwandtnerM,MildnerM, MlitzV, GruberF, EckhartL,WerfelT,GutzmerR,EliasPMandTschachlerE. Histaminesuppressesepidermalkeratinocytedifferentiationandimpairsskinbarrierfunctioninahumanskinmodel.EuropeanJournalofAllergyandClinicalimmunology(2013);68(1):37–47. 

2)Skinmodel:Neonatalkeratinocyteswereculturedinamicrofluidicsystemtopreciselyexaminethemorphology,behaviorandviABIlityofindividualcells. AdrianT.O’Neill,NancyA.Monteiro-Riviere,andGlennM.Walker.Characterizationofmicrofluidichumanepidermalkeratinocyteculture.Cytotechnology(2008);56(3):197–207. 

3)Cancer:KeratinocyteswereusedinthisstudytodemonstratethatdysregulatedactivationoftypeIinterferoninnateimmunityisimplicatedinthemolecularprocessestriggeredbyanti-EGFRdrugsandleADIngtopersistentskininflammation. CarrieHS,HongY,YunboL,JenniferSM,SrideviB,GayleneS,JudithACandThomasRS. EGFreceptorsignalingblocksarylhydrocarbonreceptor-mediatedtranscriptionandcelldifferentiationinhumanepidermalkeratinocytes.ProceedingsofNationalAcademyofSciences(2009);106(11):4266-71. 

4)HumanPapillomaviruses(HPV)mediatedskincancerresponse:Aninfectionoftheskinwithhumanpapillomaviruses(HPV)mayresultinbenigntumorswithlimitedgrowth,whichtendtoregressspontaneouslyandcausesnon-melanomaskincancerdevelopmentinepidermodysplasiaverruciformis(EV)andimmunosuppressedpatients. ThepresentstudyutilizedpooledkeratinocytesculturedinKGM^TM-GoldMediumfromseveralhealthydonors.ThedatashowedthatbetaPVmayincreasethenumberofstemcell-likecellspresentduringearlycarcinogenesisandthusenablethepersistenceandaccumulationofDNAdamagenecessarytogeneratemalignantstemcells. MartinH,AdrianB,CinziaB,MarisaG,JohnD,AlanS,HerbertP,IanMandBakiA.Expressionofbetapapillomavirusoncogenesincreasesthenumberofkeratinocyteswithstemcell-likeproperties.JournalofVirology(2013);87(22):12158-12165. 

New!PurchaseourAdultKeratinocyteCellCultureKit(catalogno.192627B)andgetadiscountedpriceoncellsandmedia.Thekitconsistsofacryopreservedampuleofadultkeratinocytes(catalogno.192627)andKGM™-GoldBulletKit™GrowthMedia(catalogno.192060)

龙沙生物技术有限公司于2019年05月10日成立。法定代表人David Johann Wach，公司经营范围包括：生物技术开发服务（我国稀有和特有的珍贵优良品种，国家保护的原产于我国的野生动、植物资源开发除外）;生物技术推广服务（我国稀有和特有的珍贵优良品种，国家保护的原产于我国的野生动、植物资源开发除外）;生物技术咨询、交流服务（我国稀有和特有的珍贵优良品种，国家保护的原产于我国的野生动、植物资源开发除外）;生物技术转让服务（我国稀有和特有的珍贵优良品种，国家保护的原产于我国的野生动、植物资源开发除外）;医学研究和试验发展（人体干细胞、基因诊断与治疗技术除外）;药品研发（人体干细胞、基因诊断与治疗技术除外）;货物进出口(涉及外资准入特别管理规定和许可审批的商品除外);技术进出口;贸易代理;商品批发贸易（涉及外资准入特别管理规定和许可审批的商品除外）;生物药品制造;化学药品原料药制造;化学药品制剂制造等。