Whenbonemarrowcellsareputintocultureunderspecificconditions,astromallayerofadherentcellsdevelopes.Thestromallayeriscomposedofmanycelltypesincludingfibroblasts,mesenchymalstemcells,
ADIpocytes,endothelialcells,andmacrophages.Thesestromalcellsfunctionasafeederlayerforhematopetic
Progenitorsandstemcellsallowingproliferationanddifferentiationofprogenitorsandstemcellstocontinueforweeksinthesecultureswithnoadditionofexogenouscytokines.Thislongtermculturesystemallowsresearcherstostudyaninvitromodelofthebonemarrowmicroenvironment,includingcell-cellinteractions,adhesionmoleculesandcytokinesecretion.Theseandmanyotherfactorsallowforthetightregulationofbloodcellproduction,progenitorcellcommitmentanddifferentiation,stemcellrenewal,andLTC-ICassays.
Availableasnon-irradiatedonly.
HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
RecommendedMedium:MyeloCult™H5100fromStemCellTechnologies,Inc.
Bonemarrowstromalcellsareoftenconfusedwithbonemarrowmesenchymalstemcells.IfyourequirethepurepopulationofbonemarrowderivedmultipotentstemcellswiththeABIlityofselfrenewalandtheabilitytodifferentiatedownmultiplelineagesincludingosteogenic,Adipogenic,andchondrogeniclineages,pleasevisitthe BoneMarrowMesenchymalStemCellpage.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.